Set a scale for the video, picture, or gel analysis.
It is helpful to have something within the movie or picture to mark the distance. For example, having a meter stick in the movie will give you a good indication of the space you are dealing with.
After you have clicked , you can drag the distance desired in your movie, then record the Distance and Units in the dialog that is displayed.
Using the meter stick example, enter 1 as the Distance and m as the Units.