Gel Analysis

Gel Electrophoresis is a method used to separate biological molecules based upon molecular charge (polarity), molecular mass, and molecular shape. This is particularly useful in separating charged biomolecules such as deoxyribonucleic acid, DNA, and ribonucleic acid, RNA. Analysis feature calculates the number of base pairs of various molecules based on the distance traveled during electrophoresis.

Setup for Gel Analysis

Choose Gel Analysis from the Insert menu, and select either Take Photo or From File. Take Photo is a live method of capturing your gel results using a ProScope, Logitech, or other digital camera. Choose From File to select a gel photograph stored in your computer.

Analyzing the Gel Photograph

  1. Click Set Origin . Position your cursor to the left of the first well and click. A yellow origin will appear where vertical and horizontal lines intersect. Drag the origin up or down to position the horizontal line in the middle of all the wells. Use the dot on the horizontal line to rotate, if needed.
  2. If your photo includes a reference to distance such as a ruler, click Set Scale . Use your cursor to draw a line of known distance on the photo. A window will appear allowing you to input the distance of the line you draw. This step is optional. If it is skipped, distances will be reported in pixels.
  3. Click Set Standard Ladder , then click the center of the first band of the Standard Ladder in the photo. Enter the number of base pairs in the window that appears. Click the next band and repeat for all bands in the standard ladder.
  4. Once the Standard Ladder has been set, click Add Lane and then the Add Lane option. Click the center of the first band of the first experimental lane. The number of base pairs will be calculated and entered in the data table and on the graph. Click on each of the remaining bands in this lane.
  5. To register the bands of the next lane, click Add Lane again. Click the Add Lane option that appears. Repeat the Step 4 procedure for each additional lane being added to your analysis of this gel photograph. Note: Logger Pro will automatically name the first experimental lane "Lane 2." If you wish to change the name, you can do so by double-clicking the name of the data set in the data set table.