Gel Analysis
Gel Electrophoresis is a method used to separate biological
molecules based upon molecular charge (polarity), molecular mass, and
molecular shape. This is particularly useful in separating charged
biomolecules such as deoxyribonucleic acid, DNA, and ribonucleic acid,
RNA. Analysis feature calculates the number of base pairs of various
molecules based on the distance traveled during electrophoresis.
Setup for Gel Analysis
Choose Gel Analysis from the Insert
menu, and select either Take Photo or From
File. Take Photo is a live method of capturing your gel
results using a ProScope, Logitech, or other digital camera. Choose
From File to select a gel photograph stored in your computer.
Analyzing the Gel Photograph
- Click Set Origin .
Position your cursor to the left of the first well and click. A yellow
origin will appear where vertical and horizontal lines intersect. Drag
the origin up or down to position the horizontal line in the middle of
all the wells. Use the dot on the horizontal line to rotate, if needed.
- If your photo includes a reference to distance such as a
ruler, click Set Scale .
Use
your cursor to draw a line of known distance on the photo. A window
will appear allowing you to input the distance of the line you draw.
This step is optional. If it is skipped, distances will be reported in
pixels.
- Click Set Standard Ladder , then click
the center of
the first band of the Standard Ladder in the photo. Enter the number of
base pairs in the window that appears. Click the next band and repeat
for all bands in the standard ladder.
- Once the Standard Ladder has been set, click Add Lane and then the Add
Lane option.
Click the center of the first band of the first experimental lane. The
number of base pairs will be calculated and entered in the data table
and on the graph. Click on each of the remaining bands in this lane.
- To register the bands of the next lane, click Add Lane
again. Click the Add Lane option that appears. Repeat the Step 4
procedure for each additional lane being added to your analysis of this
gel photograph. Note: Logger Pro
will automatically name the first experimental lane "Lane 2." If you
wish to change the name, you can do so by double-clicking the name of
the data set in the data set table.